Cytotoxicity Test of the Best Cosmetic Ingredient DCR 516 for Sensitive Skin

3月 18, 2021Cosmetic ingredients

1. DCR 516 Test Method

DCR 516 can relieve sensitive skin, such as skin stinging, skin itching, skin redness, etc. In order to prove anti-irritant cosmetic ingredient DCR516‘s safety on skin, this test is carried out on human keratinocytes. There are two test methods: cytotoxicity detection and morphological detection.

2. DCR 516 Test Result

1. MTT Result

The sample DCR516 is administered 8 dosing concentrations. MTT result is shown in Table 1, and the change trend of cell viability is shown in Figure 1.

CELL TEST Table 1 MTT Result of DCR516 Sample

CELL VIABILITY

Figure 1 Cell Viability Curve

Therefore, the MTT result shows when DCR 516’s concentration is less than 6.25 mg/mL, the cell viability is high, and DCR 516’s effect on sensitive skin is more obvious.

Based on the MTT result, 5 concentrations with cell viability around 90% are selected for morphological test.

concentration

2. DCR 516 Morphological Test Result

DCR516 no cytotoxicity When concentration of DCR516 sample is 6.25 mg/mL, the relative cell viability value is 91.23%. The cell state is not significantly different from that of the solvent control group. Therefore, according to MTT result and morphology, cosmetic raw material DCR 516 applied to sensitive skin shows no cytotoxicity within the concentration range of 6.25 mg/mL.

3. Conclusion for DCR 516’s Cytotoxicity Test

Based on keratinocytes, when concentration of cosmetic raw material DCR 516 that is benefit to sensitive skin is less than 6.25mg/mL, there is no cytotoxicity.