1. PMSoothe® 9390 Overview
PMSoothe® 9390 is a combination of Piper Methysticum Root Extract and Citrus aurantium fruit extract. Piper methysticum Forst is a perennial evergreen shrub-like medicinal plant of the Piperaceae family, widely used in nutritional supplements and herbal preparations in Europe and the USA. Piper methysticum extract is an alcohol and water extract of the dried rhizome of Piper methysticum, which effectively inhibits sensory nerve overreaction, thereby significantly increasing the skin’s tolerance to external stimuli, making it a natural cosmetic ingredient with potent analgesic and anti-irritant properties. Citrus aurantium is a plant of the citrus family Rutaceae. Its fruit is rich in free and glycosidic flavonoids, which are natural bioactive substances that help to reduce capillary fragility, increase vascular elasticity and reduce the permeability of capillary walls, while reducing the redness caused by irritation and allergy. Plamed has also demonstrated, based on neuronal and keratin-forming cell tests, that PMSoothe® 9390 has a strong action potential inhibition as well as TRPV1, which acts as an analgesic. PMSoothe® 9390 is therefore a water-soluble cosmetic ingredient with excellent neuro-relaxing properties.
| Product Name | INCI Name | ||
| PMSoothe® 9390 | PIPER METHYSTICUM ROOT EXTRACT,
HYDROLYZED CITRUS AURANTIUM DULCIS FRUIT EXTRACT, HYDROXYPROPYL CYCLODEXTRIN |
||
| Product ID | Specification | Minimal Package | Minimal Order Quality |
| PMS0201 | PMS0201 | 1KG | 1KG |
2. Botanical Source
Kava, also named Piper methysticum Forst, is a perennial, erect shrubby medicinal plant of the Piperaceae family, native to the islands of the South Pacific, wild or cultivated, the roots and rhizomes are used as medicine, the active part being the fat-soluble resinous part. The main medicinal components of kava piper are carvacrolide, kawain. Kava pepper has long been a widely used medicinal ingredient in Europe and the USA for its skin calming, local anaesthetic, antibacterial and anti-anxiety effects, and is also widely used as a food supplement. The dried rhizome is used as a raw material for cosmetic products.
Lime (scientific name: Citrus aurantiumL.), also known as bitter orange, is a small tree plant of the citrus family Rutaceae, native to China south of the southern slopes of the Qinling Mountains, and is widely used as a root-stock for grafting sweet oranges and broad-skinned tangerines. It is also used medicinally as a stomachic, fortifying, wind-repelling and orthodontic agent for colds, indigestion. The chemical composition has three main groups, namely alkaloids, flavonoids and volatile oils. It can whiten and remove blemishes, make the skin smooth and elastic, and can be used in facial cleansers to deep cleanse the skin.
3. Technical Data Sheet
| Organoleptic Tests | |
| Appearance | Off white to light yellow powder |
| Odor | Characteristic |
| Taste | Characteristic |
| Physical Characteristics | |
| Moisture | ≤10.0% |
| Ash content | ≤7.0% |
| Heavy Metals | |
| Pb | ≤10ppm |
| As | ≤2ppm |
| Hg | ≤1ppm |
| Cd | ≤5ppm |
| Microbiological Tests | |
| Total of bacteria | ≤1000cfu/g |
| Yeast & mold | ≤100cfu/g |
| Pseudomonas aeruginosa | Not detected |
| Staphylococcus aureus | Not detected |
| Heat-resistant escherichia coil | Not detected |
4. PMSoothe® 9390 Function & Mechanism
- Nerve soothing effect
- Zebrafish soothing efficacy (total exercise distance) test
Although glacial acetic acid is a weak acid, it is strongly penetrating and corrosive. Skin contact with glacial acetic acid can cause a stinging and burning sensation. Zebrafish with underdeveloped swimming ability is selected and stimulated with glacial acetic acid to cause inflammatory pain over a larger area and for a longer period of time, resulting in more movement tracks and more pain. A behavioural analyser is applied to test the total distance travelled by the zebrafish and to evaluate whether PMSoothe® 9390 has a soothing effect. The results of PMSoothe® 9390 is tested as below.
Phenotypes and bar graphs of the efficacy experiments are shown in Figures 1 and 2 below.
It was observed that the total movement distance of PMSoothe® 9390 group was significantly reduced compared to the model control group, revealing the soothing efficacy of PMSoothe® 9390, which was 40% and 90% at 0.01% and 0.2% concentrations respectively, specifically in terms of neuroleptic efficacy.
- Effect of PMSoothe® 9390 on neuronal action potentials
The dorsal root ganglion, DRG, is a primary neuron of sensory transmission, which has the function of transmitting and regulating body sensations, receiving and conducting injurious sensations. It is now an important model for neuropathic pain treatment at the isolated cellular level, and has become a hot research topic in the fields of pain mechanisms and pain treatment. A variety of ion channels exist in the dorsal root ganglion, and the one associated with pain and other diseases is the sodium channel. After nerve injury, the activity of voltage-gated sodium channels is altered, causing increased excitability of neurons and producing abnormal spontaneous firing. In this experiment, the manual diaphragm clamp technique was used to investigate the effects of PMSoothe® 9390 on various parameters of the action potential of DRG neurons in SD rats, so as to further investigate the role of primary neurons in the mechanism of pain onset. The test results were as follows.
The results showed that the sample PMSoothe® 9390 at concentrations of 0.003125% and 1.2 mg/mL significantly reduced the frequency of action potential release from neuronal cells, allowing a decrease in the frequency of excitation of nerve cells per unit time. Among them, the inhibition rate was 56.25% at the concentration of 0.003125% and 93.75% at the concentration of 1.2 mg/mL.
- Capsaicin receptor (TRPV1) protein content assay
The main objective of this assay was to evaluate the soothing effect of the test article by inhibiting TRPV1, a gated ion channel receptor, also known as the capsaicin receptor, which is a heat-sensitive receptor, by activating TRPV1 after the action of capsaicin and others, leading to further Ca2+ inward flow. After the action of the test article, TRPV1 is inhibited and the intracellular Ca2+ concentration is reduced, thus it can be shown that the test article achieves a soothing effect by inhibiting TRPV1.
The test results of PMSoothe® 9390 are as follows.
Based on keratin-forming cells, PMSoothe® 9390 showed a significant decrease in capsaicin receptor (TRPV1) protein content at 0.0078% (m/v) compared to the control, with a 26.53% inhibition rate, indicating that the samples achieved soothing effects by inhibiting capsaicin receptor (TRPV1) protein expression at this concentration.
4. Human capsaicin patch test
The experiment was divided into two groups A and B. Group A was the blank control group, 10 drops of 0.01% capsaicin were put on the patch test paper; Group B was the PMSoothe® 9390 test group, 10 drops of prepared solution containing 0.01% capsaicin and 3% PMSoothe® 9390 were put on the patch test paper, and the two groups A and B were put on the left and right arms at the same time, and the patch was torn off after 30 min, and the results were as follows.
The results showed significant stinging, burning and redness in group A, while no adverse reactions were observed in group B. PMSoothe® 9390 was confirmed to significantly improve skin tolerance.
Please scan the QR code below to watch the skin anti-irritant test video.
5. Plamed PMSoothe® 9390 Characteristic
- Water-soluble natural ingredients.
- Strong efficacy: 4-tert-butylcyclohexanol inhibitsTRPV1 protein by 28.57% at 0.0078% (the maximum safe concentration of 4-tert-butylcyclohexanol); PMSoothe® 9390 inhibits TRPV1 protein by 26.53% at the same concentration. The two effects are close to each other.
- Up to 90% nerve soothingeffect based on zebrafish experiments.
- In neuronal cell-based test, action potential release frequency is inhibited by 93.75%.
6. PMSoothe® 9390 Application & Reference Dosage
| Application Product | Reference Dosage |
| Facial cleanser, toner, essence, lotion, cream, gel, eye cream, aftershave, etc. | 0.1% ~ 3.0% |
Formulation suggestions:
- If the product contains mild ingredients, the recommended dosage is 0.1% to 1%.
- If the product contains irritating ingredients, the recommended dosage is 1% to 3% depending on the strength of the irritation.
- The recommended pH range is acidic to neutral (pH 4.0-7.0) and the temperature range is room temperature or low; when polyols are used as solvents, care should be taken to deal with precipitation.
- The precipitation will occur when the solution of low alcohol is dissolved for a long time. 5% butylene glycol will precipitate significantly when dissolved for a long time.
7. PMSoothe® 9390 Safety
- Piper Methysticum Root Extract, Citrus Aurantium Dulcis (Orange) Fruit Extract have approved by CFDA (China Food and Drug Administration) as cosmetic ingredient.
- International Cosmetic Raw Material Standard Chinese Name Directory (2015 edition) has included Piper Methysticum Root Extract, Citrus Aurantium Dulcis (Orange) Fruit Extract.
- The International Catalogue of Chinese Cosmetic Raw Materials Standards in 2021 includes Piper Methysticum Root Extract, Citrus Aurantium Dulcis (Orange) Fruit Extract.
- Plamed tested the stability of PMSoothe® 9390 for 2 months and concluded that:
- the aqueous solution does not produce precipitation under different test conditions and has good stability;
- the aqueous solution is clear and translucent when placed at 45°C, but there is slight discolouration at high temperature for a long time;
- the 5% butanediol and 10% butanediol solutions produce precipitation visible to the naked eye, while the 20% butanediol solution has good stability.
- Plamed tests PMSoothe® 9390’s 55 pesticide residues and all results are non-detect. The reference basis is GB/T39665-2020 “Determination of 55 prohibited pesticide residues in cosmetics containing plant extracts”.
8. Packing and Storage
Package: Packed in 25kg paper drums with two plastic bags inside.
Storage: Stored in a cool and dry place and away from direct sunlight and oxidizing agents.
Plamed focuses on natural cosmetic ingredients for more than 10 years. We have founded four subsidiary companies, which respectively develops different kinds of cosmetic raw material. Plamed is a company whose CEO is designated as the first secretary general of Shaanxi Plant Extraction Association.
As a professional Skin Soothing Ingredient PMSoothe® 9390 manufacturer, Plamed have been constantly upgrading the production process. We firmly believe that good Skin Soothing Ingredient PMSoothe® 9390 and good Skin Soothing Ingredient PMSoothe® 9390 price will help customers make good terminal products and help customers win a lasting and broad market.
